Microtopographies control the development of basal protrusions in epithelial sheets
TypeArticles dans des revues avec comité de lecture
Cells are able to develop various types of membrane protrusions that modulate their adhesive, migratory, or functional properties. However, their ability to form basal protrusions, particularly in the context of epithelial sheets, is not widely characterized. The authors built hexagonal lattices to probe systematically the microtopography-induced formation of epithelial cell protrusions. Lattices of hexagons of various sizes (from 1.5 to 19 μm) and 5-10 μm height were generated by two-photon photopolymerization in NOA61 or poly(ethylene glycol) diacrylate derivatives. The authors found that cells generated numerous, extensive, and deep basal protrusions for hexagons inferior to cell size (3-10 μm) while maintaining a continuous epithelial layer above structures. They characterized the kinetics of protrusion formation depending on scaffold geometry and size. The reported formation of extensive protrusions in 3D microtopography could be beneficial to develop new biomaterials with increased adhesive properties or to improve tissue engineering.
Files in this item
Showing items related by title, author, creator and subject.
MAZEL, Vincent; GUERARD, Sandra; CROQUELOIS, Benjamin; KOPP, Jean-Benoit; GIRARDOT, Jérémie; DIARRA, Harona; BUSIGNIES, Virginie; TCHORELOFF, Pierre (Elsevier, 2016)Mechanical strength is an important critical quality attribute for tablets. It is classically measured, in the pharmaceutical field, using the diametral compression test. Nevertheless, due to small contact area between the ...
Zn1-xMgxO thin films deposited by original Infrared assisted Spray Chemical Vapor Deposition: evaluation of structural, optical and electrical properties with the aim of provide Cu(In,Ga)Se2 based photovoltaic devices FROGER, Vincent; DABOS-SEIGNON, Sylvie; NOËL, Sophie; CHAPRON, David; BOUTEVILLE, Anne (INOE Publishing House, 2014)In this work, magnesium doped zinc oxide (Zn1-xMgxO) thin films were deposited using an original infrared assisted Spray Chemical Vapor Deposition (Spray-CVD) technique on borosilicate glass substrates. With a simple, safe ...
BENOIT, Aurélie; GUERARD, Sandra; GILLET, Brigitte; GUILLOT, Geneviève; HILD, François; MITTON, David; PERIE, Jean Noël; ROUX, Stéphane (elsevier, 2009)A mini-compression jig was built to perform in situ tests on bovine trabecular bone monitored by micro-MRI. The MRI antenna provided an isotropic resolution of 78 microm that allows for a volume correlation method to be ...
Thermal behavior of poly(VDF-ter-TrFE-ter-CTFE) copolymers: Influence of CTFE termonomer on the crystal-crystal transitions BARGAIN, François; THUAU, Damien; PANINE, Pierre; HADZIIOANNOU, Georges; DOMINGUES DOS SANTOS, Fabrice; TENCE-GIRAULT, Sylvie (elsevier, 2019)Depending on their CTFE content (from 0 to 10 mol %), poly (VDF-ter-TrFE-ter-CTFE), poly(vinylidene fluoride-ter-trifluoroethylene-ter-chlorotrifluoroethylene) copolymers exhibit ferroelectric (FE) or relaxor ferroelectric ...
On the Accuracy of Elastic Strain Field Measurements by Laue Microdiffraction and High-Resolution EBSD: a Cross-Validation Experiment PLANCHER, Emeric; PETIT, Johann; MAURICE, Claire; FAVIER, Véronique; SAINTOYANT, Lucie; LOISNARD, Dominique; RUPIN, N.; MARIJON, Jean Baptiste; ULRICH, Olivier; BORNERT, Michel; MICHA, Jean Sébastien; ROBACH, Odile; CASTELNAU, Olivier (Springer New York LLC, 2016)Determining the accuracy of elastic strain measurements in plastically deformed alloys is an experimental challenge. To develop a novel cross-validation procedure, a controlled elasto-plastic strain gradient was created ...