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A mesofluidic multiplex immunosensor for detection of circulating cytokeratin-positive cells in the blood of breast cancer patients

Article dans une revue avec comité de lecture
Author
BRETON, François
1798 Institut d'Alembert [IDA]
BENNETAU, Bernard
775 Laboratoire de chimie organique et organométallique [LCOO]
24493 Institut des Sciences Moléculaires [ISM]
LIDEREAU, Rosette
24493 Institut des Sciences Moléculaires [ISM]
THOMAS, Laurent
3093 Genetique Moleculaire des Cancers d'Origine Epitheliale
EHRHART, Jean-Claude
197920 Stabilité Génétique et Oncogenèse [UMR 8200]
TAUC, Patrick
1257 Laboratoire de Biologie et de Pharmacologie Appliquée [LBPA]
TRAN, Phuong-Lan
1798 Institut d'Alembert [IDA]
ccREGNIER, Gilles
86289 Laboratoire Procédés et Ingénierie en Mécanique et Matériaux [PIMM]

URI
http://hdl.handle.net/10985/17716
DOI
10.1007/s10544-010-9465-7
Date
2011
Journal
Biomedical Microdevices

Abstract

We have recently reported the analytical performance of an immunosensor comprising one mm-scale parallel plate laminar flow chamber and applied to capture MCF7 breast cancer cells (Ehrhart et al., Biosens. Bioelectr. 24, 467, 2008). Herein we present a new multiplex immunosensor embodying four parallel plate laminar flow chambers that fit onto a standard, functionalized, microscopy glass slide. The four surfaces are coated with long alkyl chain spacers of 21-aminohenicosyl trichlorosilane (AHTS) and then grafted with a monoclonal anti-human epithelial cell adhesion molecule (EpCAM) antibody specific of target cells to immobilize. We first demonstrate a significantly (P < 0.01) improved capacity of each of the four flow chambers of the multiplex immunosensor to capture MCF7 cells compared to the previous single chamber device. Second, in addition to an increase of cell immobilization, the multiplex device offers a versatile tool easily grafted with various purified antibodies onto the four surfaces. Third, we obtained high cell capture rate and efficiency of various numbers of MCF7 cells spiked in buffer containing an equal number of background leukocytes. And fourth, we demonstrate isolation efficiency of circulating tumor cells (CTCs) from peripheral blood drawn from a small cohort of patients with localized or metastatic breast cancer. This new multiplex immunosensor could be tested for its potential to capture different subpopulations of CTCs.

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