The CryoCapsule : Simplifying Correlative Light to Electron Microscopy
Article dans une revue avec comité de lecture
Correlating complementary multiple scale images of the same object is a straightforward means to decipher biological processes. Light microscopy and electron microscopy are the most commonly used imaging techniques, yet despite their complementarity, the experimental procedures available to correlate them are technically complex. We designed and manufactured a new device adapted to many biological specimens, the CryoCapsule, that simplifies the multiple sample preparation steps, which at present separate live cell fluorescence imaging from contextual high-resolution electron microscopy, thus opening new strategies for full correlative light to electron microscopy. We tested the biological application of this highly optimized tool on three different specimens: the in vitro Xenopus laevis mitotic spindle, melanoma cells over-expressing YFP-langerin sequestered in organized membranous subcellular organelles and a pigmented melanocytic cell in which the endosomal system was labeled with internalized fluorescent transferrin.
Showing items related by title, author, creator and subject.
Relevance of using a compressive preload in the cervical spine : an experimental and numerical simulating investigation Article dans une revue avec comité de lectureBARREY, Cédric; ROUSSEAU, Marc-Antoine; PERSOHN, Sylvain; CAMPANA, Sophie; PERRIN, Gilles; SKALLI, Wafa (Springer-Verlag, 2015)Simulating compressive action of muscles, a follower load attends to reproduce a more physiological biomechanical behaviour of the cervical spine. Only few experimental studies reported its influence on kinematics and ...
A mesofluidic multiplex immunosensor for detection of circulating cytokeratin-positive cells in the blood of breast cancer patients Article dans une revue avec comité de lectureBRETON, François; BENNETAU, Bernard; LIDEREAU, Rosette; THOMAS, Laurent; REGNIER, Gilles; EHRHART, Jean-Claude; TAUC, Patrick; TRAN, Phuong-Lan (Springer Verlag, 2011)We have recently reported the analytical performance of an immunosensor comprising one mm-scale parallel plate laminar flow chamber and applied to capture MCF7 breast cancer cells (Ehrhart et al., Biosens. Bioelectr. 24, ...
Reuse of medical face masks in domestic and community settings without sacrificing safety: Ecological and economical lessons from the Covid-19 pandemic Article dans une revue avec comité de lectureALCARAZ, Jean-Pierre; LE COQ, Laurence; POURCHEZ, Jérémie; THOMAS, Dominique; CHAZELET, Sandrine; BOUDRY, Isabelle; BARBADO, Maud; SILVENT, Sophie; DESSALE, Claire; ANTOINE, Fabienne; GUIMIER-PINGAULT, Catherine; CORTELLA, Laurent; ROUIF, Sophie; BARDIN-MONNIER, Nathalie; CHARVET, Augustin; DUFAUD, Olivier; LECLERC, Lara; MONTIGAUD, Yoann; LAURENT, Coralie; VERHOEVEN, Paul; JOUBERT, Aurélie; BOUHANGUEL, Ala; ANDRES, Yves; GAFFÉ, Joël; MARTIN, Donald K.; HUET, Christophe; BOISSET, Sandrine; MAURIN, Max; RUMEAU, Pascal; CHARLOT, Frédéric; RICHAUD, Emmanuel; MOREAU-GAUDRY, Alexandre; BONNETERRE, Vincent; CINQUIN, Philippe; LANDELLE, Caroline (Elsevier, 2021)The need for personal protective equipment increased exponentially in response to the Covid-19 pandemic. To cope with the mask shortage during springtime 2020, a French consortium was created to find ways to reuse medical ...
BrevetD'ACUNTO, Alain; MARTIN, Patrick; MIHALUTA, Marius; PERRIN, Henri-François; BETTEGA, Louis; CAUCHOIS, Jean-Pierre; CINI, Jérôme; PRADELLA, Serge (2010)The invention relates to a device (IO) for ma.king a part of a composite material by resin-transfer moulding, that includes a manufacturing mould (IO) in which is placed a blank (1) of the part to be impregnated with resin, ...
Article dans une revue avec comité de lectureBERTHE, Laurent; ARRIGONI, Michel; BOUSTIE, Michel; CUQ-LELANDAIS, Jean Paul; BROUSSILLOU, Cédric; FABRE, Grégory; JEANDIN, Michel; GUIPONT, Vincent; NIVARD, Mariette (Taylor & Francis, 2011)This paper proposes a state-of-the-art laser adhesion test. It consists of testing material interfaces with laser-driven shock wave. Since the first demonstration in the 1980s by Vossen, many studies and developments have ...