The CryoCapsule : Simplifying Correlative Light to Electron Microscopy
TypeArticles dans des revues avec comité de lecture
Correlating complementary multiple scale images of the same object is a straightforward means to decipher biological processes. Light microscopy and electron microscopy are the most commonly used imaging techniques, yet despite their complementarity, the experimental procedures available to correlate them are technically complex. We designed and manufactured a new device adapted to many biological specimens, the CryoCapsule, that simplifies the multiple sample preparation steps, which at present separate live cell fluorescence imaging from contextual high-resolution electron microscopy, thus opening new strategies for full correlative light to electron microscopy. We tested the biological application of this highly optimized tool on three different specimens: the in vitro Xenopus laevis mitotic spindle, melanoma cells over-expressing YFP-langerin sequestered in organized membranous subcellular organelles and a pigmented melanocytic cell in which the endosomal system was labeled with internalized fluorescent transferrin.
Showing items related by title, author, creator and subject.
D'ACUNTO, Alain; MARTIN, Patrick; MIHALUTA, Marius; PERRIN, Henri-François; BETTEGA, Louis; CAUCHOIS, Jean-Pierre; CINI, Jérôme; PRADELLA, Serge (2010)The invention relates to a device (IO) for ma.king a part of a composite material by resin-transfer moulding, that includes a manufacturing mould (IO) in which is placed a blank (1) of the part to be impregnated with resin, ...
GUENOUN, Gabriel; FAOU, Jean-Yvon; REGNIER, Gilles; SCHMITT, Nicolas (elsevier, 2019)Cold uniaxial pressed PTFE compacts were subjected to thermal cycles of increasing maximum temperature below melting temperature, and thermal expansion was followed in both the compaction and transverse directions. The ...
VINCENT, Jean-Paul; BAUDOUIN, Cyrille; BRUYERE, Jérôme; DANTAN, Jean-Yves; BIGOT, Régis (World Scientific Publishing, 2009)Tolerance verification permits to check the product conformity and to verify assumptions made by the designer. For conformity assessment, the uncertainty associated with the values of the measurands must be known. In the ...
LÉON, Jean-Claude; DUPEUX, Thomas; CHARDONNET, Jean-Rémy; PERRET, Jérôme (ASME, 2016)The simulation of grasping operations in virtual reality (VR) is required for many applications, especially in the domain of industrial product design, but it is very difficult to achieve without any haptic feedback. Force ...
LEON, Jean-Claude; DUPEUX, Thomas; CHARDONNET, Jean-Rémy; PERRET, Jérôme (ASME, 2015)Achieving grasping tasks in real time with haptic feedback may require the control of a large number of degrees of freedom (DOFs) to model hand and finger movements. This is mandatory to grasp objects with dexterity. Here, ...