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A Novel Antibacterial Compound Decreases MRSA Biofilm Formation Without the Use of Antibiotics in A Murine Model

Article dans une revue avec comité de lecture
Author
ccBOULOUSSA, Houssam
DURAND, Zoe
ccGIBON, Emmanuel
CHEN, Antonia F.
538529 Brigham & Women’s Hospital [Boston] [BWH]
GRANT, Matthew
SALEH‐MGHIR, Azzam
470602 Infection et inflammation [2I]
MIRZA, Mohsin
STUTZMAN, Bradley
ccVERGARI, Claudio
1001017 Institut de Biomécanique Humaine Georges Charpak [IBHGC]
YUE, James
ccANZALA, Nelson
BONNOT, Dorian
ALBAC, Sandrine
BOULOUSSA, Othman
ccCROISIER, Delphine
35051 CHU Dijon
300924 Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand [CHU Dijon]
497166 Service de Maladies Infectieuses et Tropicales [CHU Dijon]
523578 Vivexia S.A.R.L. [Gemeaux]

URI
http://hdl.handle.net/10985/24101
DOI
10.1002/jor.25638
Date
2023-06
Journal
Journal of Orthopaedic Research

Abstract

Despite significant advancements in material science, surgical site infection (SSI) rates remain high and prevention is key. This study aimed to demonstrate the in-vivo safety and antibacterial efficacy of titanium implants treated with a novel broad-spectrum biocidal compound (DBG21) against methicillin-resistant Staphylococcus aureus (MRSA). Titanium (Ti) discs were covalently bound with DBG21. Untreated Ti discs were used as controls. All discs were implanted either untreated for 44 control mice or DBG21-treated for 44 treated mice. After implantation, 1x107 colony forming units (CFU) of MRSA were injected into the operating site. Mice were sacrificed at day 7 and 14 to determine the number of adherent bacteria (biofilm) on implants and in the peri-implant surrounding tissues. Systemic and local toxicity were assessed. At both 7 and 14 days, DBG21-treated implants yielded a significant decrease in MRSA biofilm (3.6 median log10 CFU (99.97%) reduction (p<0.001) and 1.9 median log10 CFU (98.7%) reduction (p=0.037), respectively) and peri-implant surrounding tissues (2.7 median log10 CFU/g (99.8%) reduction (p<0.001) and 5.6 median log10 CFU/g (99.9997%) reduction (p<0.001), respectively). There were no significant differences between control and treated mice in terms of systemic and local toxicity. DBG-21 demonstrated a significant decrease in the number of biofilm bacteria without associated toxicity in a small animal implant model of SSI. Preventing biofilm formation has been recognized as a key element of preventing implant-related infections.

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