A Novel Antibacterial Compound Decreases MRSA Biofilm Formation Without the Use of Antibiotics in A Murine Model
Article dans une revue avec comité de lecture
Author
Date
2023-06Journal
Journal of Orthopaedic ResearchAbstract
Despite significant advancements in material science, surgical site infection (SSI) rates remain high and prevention is key. This study aimed to demonstrate the in-vivo safety and antibacterial efficacy of titanium implants treated with a novel broad-spectrum biocidal compound (DBG21) against methicillin-resistant Staphylococcus aureus (MRSA). Titanium (Ti) discs were covalently bound with DBG21. Untreated Ti discs were used as controls. All discs were implanted either untreated for 44 control mice or DBG21-treated for 44 treated mice. After implantation, 1x107 colony forming units (CFU) of MRSA were injected into the operating site. Mice were sacrificed at day 7 and 14 to determine the number of adherent bacteria (biofilm) on implants and in the peri-implant surrounding tissues. Systemic and local toxicity were assessed. At both 7 and 14 days, DBG21-treated implants yielded a significant decrease in MRSA biofilm (3.6 median log10 CFU (99.97%) reduction (p<0.001) and 1.9 median log10 CFU (98.7%) reduction (p=0.037), respectively) and peri-implant surrounding tissues (2.7 median log10 CFU/g (99.8%) reduction (p<0.001) and 5.6 median log10 CFU/g (99.9997%) reduction (p<0.001), respectively). There were no significant differences between control and treated mice in terms of systemic and local toxicity. DBG-21 demonstrated a significant decrease in the number of biofilm bacteria without associated toxicity in a small animal implant model of SSI. Preventing biofilm formation has been recognized as a key element of preventing implant-related infections.
Files in this item
- Name:
- IBHGC_JOR_2023_VERGARI.pdf
- Size:
- 5.207Mb
- Format:
- Description:
- A novel antibacterial compound ...
Related items
Showing items related by title, author, creator and subject.
-
Article dans une revue avec comité de lecturePIETTON, Raphael; BOULOUSSA, Houssam; SKALLI, Wafa; VIALLE, Raphaël; VERGARI, Claudio (Lippincott, Williams & WilkinsWolters kluwer, 2020)Study design: A reproducibility study of preoperative rib cage 3D measurements was conducted for patients with Adolescent Idiopathic Scoliosis (AIS). Objective: to assess the reliability of rib cage 3D reconstructions using ...
-
Article dans une revue avec comité de lectureCOURTOIS, Isabelle; EBERMEYER, Eric; PIETTON, Raphaël; BOULOUSSA, Houssam; VIALLE, Raphaël; SKALLI, Wafa; VERGARI, Claudio (Springer Verlag, 2019)Purpose: To determine the short-term effect of bracing of adolescent idiopathic scoliotic (AIS) patients on the relationships between spinopelvic parameters related to balance, by comparing their in and out-of-brace geometry ...
-
Article dans une revue avec comité de lectureCOURTOIS, Isabelle; EBERMEYER, Eric; VIALLE, Raphaël; BOULOUSSA, Houssam; SKALLI, Wafa; VERGARI, Claudio (Springer Verlag, 2016)Purpose Personalized modeling of brace action have potential in improving brace efficacy in adolescent idiopathic scoliosis (AIS). Model validation and simulation uncertainty are rarely addressed, limiting the clinical ...
-
Article dans une revue avec comité de lectureBOULOUSSA, Houssam; HAEN, Thomas-Xavier; SKALLI, Wafa; VIALLE, Raphaël; VERGARI, Claudio (Springer Verlag, 2019)Purpose Various spinal and rib cage parameters measured from complex examinations were found to be correlated with preoperative pulmonary function tests (PFT). The aim was to investigate the relationship between preoperative ...
-
Article dans une revue avec comité de lecturePIETTON, Raphaël; BOULOUSSA, Houssam; LANGLAIS, Tristan; TAYTARD, Jessica; BEYDON, Nicole; SKALLI, Wafa; VIALLE, Raphaël; VERGARI, Claudio (The British Editorial Society of Bone & Joint Surgery, 2022)Aims This study addressed two questions: first, does surgical correction of an idiopathic scoliosis increase the volume of the rib cage, and second, is it possible to evaluate the change in lung function after corrective ...