• français
    • English
    English
  • Ouvrir une session
Aide
Voir le document 
  •   Accueil de SAM
  • Institut de Mécanique et d’Ingénierie de Bordeaux (I2M)
  • Voir le document
  • Accueil de SAM
  • Institut de Mécanique et d’Ingénierie de Bordeaux (I2M)
  • Voir le document
JavaScript is disabled for your browser. Some features of this site may not work without it.

Rapid Biofabrication of an Advanced Microphysiological System Mimicking Phenotypical Heterogeneity and Drug Resistance in Glioblastoma

Article dans une revue avec comité de lecture
Auteur
ccPUN, Sirjana
ccPRAKASH, Anusha
107363 University of Cincinnati [UC]
DEMAREE, Dalee
107363 University of Cincinnati [UC]
KRUMMEL, Daniel Pomeranz
ccSCIUME, Giuseppe
1002421 Institut de Mécanique et d'Ingénierie de Bordeaux [I2M]
SENGUPTA, Soma
44261 University of North Carolina [Chapel Hill] [UNC]
BARRILE, Riccardo

URI
http://hdl.handle.net/10985/25648
DOI
10.1002/adhm.202401876
Date
2024-08-05
Journal
Advanced Healthcare Materials

Résumé

AbstractMicrophysiological systems (MPSs) reconstitute tissue interfaces and organ functions, presenting a promising alternative to animal models in drug development. However, traditional materials like polydimethylsiloxane (PDMS) often interfere by absorbing hydrophobic molecules, affecting drug testing accuracy. Additive manufacturing, including 3D bioprinting, offers viable solutions. GlioFlow3D, a novel microfluidic platform combining extrusion bioprinting and stereolithography (SLA) is introduced. GlioFlow3D integrates primary human cells and glioblastoma (GBM) lines in hydrogel‐based microchannels mimicking vasculature, within an SLA resin framework using cost‐effective materials. The study introduces a robust protocol to mitigate SLA resin cytotoxicity. Compared to PDMS, GlioFlow3D demonstrated lower small molecule absorption, which is relevant for accurate testing of small molecules like Temozolomide (TMZ). Computational modeling is used to optimize a pumpless setup simulating interstitial fluid flow dynamics in tissues. Co‐culturing GBM with brain endothelial cells in GlioFlow3D showed enhanced CD133 expression and TMZ resistance near vascular interfaces, highlighting spatial drug resistance mechanisms. This PDMS‐free platform promises advanced drug testing, improving preclinical research and personalized therapy by elucidating complex GBM drug resistance mechanisms influenced by the tissue microenvironment.

Fichier(s) constituant cette publication

Nom:
I2M-AHM-Sciume-2024.pdf
Taille:
5.415Mo
Format:
PDF
Voir/Ouvrir
CC BY-NC
Ce document est diffusé sous licence CC BY-NC

Cette publication figure dans le(s) laboratoire(s) suivant(s)

  • Institut de Mécanique et d’Ingénierie de Bordeaux (I2M)

Documents liés

Visualiser des documents liés par titre, auteur, créateur et sujet.

  • Digital twinning of Cellular Capsule Technology: Emerging outcomes from the perspective of porous media mechanics 
    Article dans une revue avec comité de lecture
    URCUN, Stéphane; ccSKALLI, Wafa; NASSOY, Pierre; BORDAS, Stéphane Pierre Alain; SCIUMÈ, Giuseppe; ccROHAN, Pierre-Yves (Public Library of Science (PLoS), 2021)
    Spheroids encapsulated within alginate capsules are emerging as suitable in vitro tools to investigate the impact of mechanical forces on tumor growth since the internal tumor pressure can be retrieved from the deformation ...
  • Cortex tissue relaxation and slow to medium load rates dependency can be captured by a two-phase flow poroelastic model 
    Article dans une revue avec comité de lecture
    URCUN, Stéphane; ROHAN, Pierre-Yves; SCIUMÈ, Giuseppe; BORDAS, Stéphane P.A. (Elsevier BV, 2021)
    This paper investigates the complex time-dependent behavior of cortex tissue, under adiabatic condition, using a two-phase flow poroelastic model. Motivated by experiments and Biot’s consolidation theory, we tackle ...
  • Numerical investigation of the time-dependent stress–strain mechanical behaviour of skeletal muscle tissue in the context of pressure ulcer prevention 
    Article dans une revue avec comité de lecture
    LAVIGNE, T.; ccSCIUMÈ, Giuseppe; ccLAPORTE, Sébastien; ccPILLET, Helene; URCUN, Stéphane; WHEATLEY, B.; ccROHAN, Pierre-Yves (Elsevier BV, 2022)
    Background Pressure-induced tissue strain is one major pathway for Pressure Ulcer development and, especially, Deep Tissue Injury. Biomechanical investigation of the time-dependent stress–strain mechanical behaviour of ...
  • Cortex tissue relaxation and slow to medium load rates dependency can be captured by a two-phase flow poroelastic model 
    Article dans une revue avec comité de lecture
    URCUN, Stéphane; SCIUMÈ, Giuseppe; BORDAS, Stéphane P.A.; ccROHAN, Pierre-Yves (Elsevier BV, 2021)
    This paper investigates the complex time-dependent behavior of cortex tissue, under adiabatic condition, using a two-phase flow poroelastic model. Motivated by experiments and Biot’s consolidation theory, we tackle ...
  • Oncology and mechanics: Landmark studies and promising clinical applications 
    Article dans une revue avec comité de lecture
    ccURCUN, Stéphane; LORENZO, Guillermo; ccBAROLI, Davide; ccROHAN, Pierre-Yves; ccSCIUME, Giuseppe; ccSKALLI, Wafa; ccLUBRANO, Vincent; BORDAS, Stéphane Pierre Alain (Elsevier, 2022-06)
    Clinical management of cancer has continuously evolved for several decades. Biochemical, molecular, and genomics approaches have brought and still bring numerous insights into cancerous diseases. It is now accepted that ...

Parcourir

Tout SAMLaboratoiresAuteursDates de publicationCampus/InstitutsCe LaboratoireAuteursDates de publicationCampus/Instituts

Lettre Diffuser la Science

Dernière lettreVoir plus

Statistiques de consultation

Publications les plus consultéesStatistiques par paysAuteurs les plus consultés

ÉCOLE NATIONALE SUPERIEURE D'ARTS ET METIERS

  • Contact
  • Mentions légales

ÉCOLE NATIONALE SUPERIEURE D'ARTS ET METIERS

  • Contact
  • Mentions légales