In-vitro cartilage growth: macroscopic mass transport modelling in a three-phase system
Communication sans acte
Transplantation of engineered tissues is of major interest as an alternative to autogenic alogenic or exogenic grafts. In this study, in vitro cartilage cell culture on a fibrous biodegradable polymer scaffold is under concern. The scaffold is first seeded with cells which adhere to the fibres and the system is then grown in a bioreactor. As reported in the literature, hydrodynamics and transport of nutrients and metabolic products during this growth process is of considerable importance, motivating our analysis. A one-equation macroscopic model was first developed in order to describe macroscopic mass transport during in vitro tissue growth using the volume averaging method. This model takes into account a three phase system composed of solid fibres, cell phase and fluid phase and allows determination of the macroscopic quantities as a function of microscopic properties and geometry at any stage of growth. In a second step, numerical tools for the computation of the effective properties were developed and validated. This validation is carried out using results available in the literature for some sub-classes of our model (namely, diffusion, diffusion/reaction and diffusion/advection problems in 2D systems). The behaviour of the macroscopic dispersion tensor for the complete model (diffusion/reaction/advection) in a three phase configuration is studied and the influence of different parameters such as the volume fractions of the phases, Peclet and Kinetic numbers is discussed.
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Conférence invitéeAHMADI-SENICHAULT, Azita; LASSEUX, Didier; LETELLIER, Samuel (2007)Flow and transport in fibrous media are encountered in a wide variety of domains ranging from biotechnology to filtration in chemical engineering. The context of this work is the in vitro cartilage cell culture on a fibrous ...
Communication avec acteLETELLIER, Samuel; LASSEUX, Didier; AHMADI-SENICHAULT, Azita (2007)Tissue engineering is of major importance in biomedical transplantation techniques. However, some questions subsist as for example the mass transport between each pahse (cell, fluide and solid). In a previous paper, a ...
Communication sans acteLASSEUX, Didier; AHMADI-SENICHAULT, Azita; ABBASIAN ARANI, Ali Akbar (2010)This work focuses on the stationary one-phase Newtonian flow in a class of homogeneous porous media at large enough flow rates leading to a non-linear relationship between the filtration velocity and the pressure gradient. ...
Conférence invitéeAHMADI-SENICHAULT, Azita; ABBASIAN ARANI, Ali Akbar; LASSEUX, Didier (2010)Significant inertial effects are observed for many applications such as flow in the near-wellbore region, in very permeable reservoirs or in packed-bed reactors. In these cases, the classical description of two-phase flow ...
Communication sans acteLASSEUX, Didier; AHMADI-SENICHAULT, Azita; ABBASIAN ARANI, Ali Akbar (2012)Our interest in this work is dedicated to the dependence upon the filtration velocity (or Reynolds number) of the inertial correction to Darcy's law for one-phase flow in homogeneous porous media. The starting point of our ...