Multiphoton imaging and Raman spectroscopy of the bovine vertebral endplate
Article dans une revue avec comité de lecture
Abstract
The interface between the intervertebral disc and the vertebral body is important to the discs’ biomechanics and physiology, and is widely implicated in its pathology. This study aimed to explore biochemically and structurally the bony endplate, cartilage endplate and intervertebral disc, below the nucleus and below the annulus in healthy bovine tails. Multiphoton imaging and spontaneous Raman spectroscopy were employed. Raman spectroscopy provided relative quantification of mineral and matrix components across the vertebral endplate and its adjacent areas with microscopic spatial resolution. Microscopy utilising second-harmonic generation (SHG) and two-photon fluorescence (TPF) allowed for the structural identification of distinct endplate regions. The cartilage endplate was revealed as structurally distinct from both the bone and disc, supporting its biomechanical function as a transition zone between the soft and hard tissue components. The collagen fibres were continuous across the tidemark which defines the interface between the mineralised and non-mineralised regions of the endplate. Raman spectroscopy revealed gradients in phosphate and carbonate content through the depth of the endplate and also differences beneath the nucleus and annulus consistent with a higher rate of remodelling under the annulus.
Files in this item
Related items
Showing items related by title, author, creator and subject.
-
Article dans une revue avec comité de lectureLANGLAIS, Tristan; DESPRAIRIES, Pierre; PIETTON, Raphaël; ROHAN, Pierre-Yves; DUBOUSSET, Jean; MEAKIN, Judith R.; WINLOVE, Peter C.; VIALLE, Raphaël; SKALLI, Wafa; VERGARI, Claudio (Springer Verlag, 2019)The main function of the intervertebral disc is biomechanical function, since it must resist repetitive high loadings, while giving the spine its flexibility and protecting the spinal cord from over-straining. It partially ...
-
Article dans une revue avec comité de lecturePEDERSEN, Peter H.; VERGARI, Claudio; TRAN, Alexia; XAVIER, Fred; JAEGER, Antoine; LABOUDIE, Pierre; HOUSSET, Victor; EISKJAER, Soren; VIALLE, Raphaël (2019)STUDY DESIGN: This was a prospective validation study with technical notes. OBJECTIVE: This study aimed to validate a new ultra-low-dose full-spine protocol for reproducible Cobb angle measurements-the "nano-dose" protocol. ...
-
Article dans une revue avec comité de lectureWANG, J.-Y.; MANSFIELD, J.C.; BRASSELET, S.; VERGARI, Claudio; MEAKIN, Judith R.; WINLOVE, Peter C. (Elsevier, 2021)Needle injection has been widely used in spinal therapeutic or diagnostic processes, such as discography. The use of needles has been suspected in causing mild disc degeneration which can lead to long-term back pain. ...
-
Article dans une revue avec comité de lecturePEDERSEN, Peter H.; VERGARI, Claudio; ALZAKRI, Abdulmajeed; VIALLE, Raphaël; SKALLI, Wafa (Elsevier, 2019)Purpose The aim of this study was to validate the reproducibility of 3D reconstructions of the spine using a new reduced micro-dose protocol. Methods First, semi-quantitative image analysis was performed using an ...
-
Article dans une revue avec comité de lectureVERGARI, Claudio; AUBERT, Benjamin; LALLEMANT-DUDEK, Pauline; HAEN, Thomas-Xavier; SKALLI, Wafa (Taylor & Francis, 2020)Methods to reconstruct anatomical structures in 3D are gaining interest in medicine because they give access to quantitative information on the patient’s geometry. However, these methods are user-dependent and require a ...